cell biolabs VPK-156說(shuō)明書(shū)

QuickTiter™ MuLV Core Antigen ELISA Kit

產(chǎn)品描述：

將一種抗mulv p30單克隆包衣抗體吸附在微量滴定板上。樣品或標(biāo)準(zhǔn)品中存在的mulv核心抗原（p30）與吸附在板上的抗體結(jié)合；添加抗mulv p30多克隆抗體并與第一抗體捕獲的抗原結(jié)合。在孵育和洗滌步驟后，添加一種HRP結(jié)合的二級(jí)抗體并結(jié)合到抗mulv p30多克隆。在洗滌過(guò)程中去除未結(jié)合的HRP結(jié)合的二級(jí)抗體，并將與HRP反應(yīng)的底物溶液加入到井中。有色產(chǎn)物的形成與樣品中存在的mulv核心抗原的量成比例。通過(guò)添加酸終止反應(yīng)，并在450 nm處測(cè)量吸光度。用重組mulv p30核心抗原制備標(biāo)準(zhǔn)曲線，測(cè)定樣品濃度。

檢測(cè)方法：Colorimetric

檢測(cè)樣本：MuLV samples

試劑盒組成：

Box 1 (shipped at room temperature)

Anti-MuLV p30 Antibody Coated Plate                  96wells

Anti-MuLV p30 Polyclonal Antibody                    20 ul

Secondary Antibody, HRP Conjugate                    20 ul

Assay Diluent 50 ml

Triton X-100 Solution                       50 ml

10X Wash Buffer                                    100 ml

Substrate Solution                                    12 ml

Stop Solution                                        12ml

Substrate Solution                                    12ml

Stop Solution                                        12ml

Box 2 (shipped on blue ice packs)

Recombinant MuLV p30 Standard                        100 ul

保存條件：Upon receipt, aliquot and store the Recombinant MuLV p30 Standard at -20oC to avoid multiple freeze/thaw cycles.  Store all other kit components at 4oC.

標(biāo)準(zhǔn)曲線圖：

MuLV Core Antigen ELISA Standard Curve.

Purification of Recombinant MuLV p30 Protein. Lane 1: MW STDs; Lane 2: E.Coli Whole Lysate (-IPTG); Lane 3: E.Coli Whole Lysate (+IPTG); Lane 4: Crude Lysate; Lane 5: Lysate after Ni-NTA beads; Lane 6: Bead Wash; Lane 7: Elution Fraction for Recombinant MuLV p30 Standard.