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CIA 膠原誘導(dǎo)的關(guān)節(jié)炎模型

更新時(shí)間：2012-08-31 點(diǎn)擊次數(shù)：7802

膠原誘導(dǎo)的關(guān)節(jié)炎模型（CIA）

實(shí)驗(yàn)方案
1. 牛II型膠原溶解在0.05mol/ml的乙酸中，濃度為2mg/ml，攪拌4°C過(guò)夜。
2. 注射前，II膠原溶液與同體積的IFA在冰浴中*乳化。
3. *天，每只大鼠尾根部皮內(nèi)注射0.1ml CII/IFA乳化劑（濃度為1mg/ml）。
4. 第7天，每只大鼠以同樣的方式再次注射。
5. 注射后，大鼠關(guān)節(jié)炎指數(shù)根據(jù)肉眼評(píng)分系統(tǒng)每周兩次進(jìn)行評(píng)價(jià)。
0－正常
1－一個(gè)足爪腫脹和/或發(fā)紅
2－2個(gè)相關(guān)關(guān)節(jié)腫脹和/或發(fā)紅
3－>2個(gè)相關(guān)關(guān)節(jié)腫脹和/或發(fā)紅
4－整個(gè)足爪嚴(yán)重炎癥
每只大小鼠關(guān)節(jié)炎指數(shù)為每只動(dòng)物4足爪得分之和。
6. 腫脹嚴(yán)重性根據(jù)器官充滿度測(cè)定法通過(guò)氣道容積的改變來(lái)測(cè)定，一周兩次。

Brief protocol of Collagen induced arthritis model （CIA）
1. Bovine CII is dissolved in 0.05mol/L acetic acid at a concentration of 2 mg/ml by stirring overnight at 4°C.
2. Before injection, CII is emulsified with an equal volume of IFA in an ice-cold water bath.
3. On day1, each rat is injected intradermally at the base of the tail with 0.1ml CII/IFA the emulsion （1mg/ml）.
4. On day 21, a second injection of CII in IFA will be administered using the same amount.
5. After the second injection，rats will be evaluated daily for arthritis using a macroscopic scoring system of 0–4.
0 no signs of arthritis
1 swelling and/or redness of the paw or 1 digit
2=2 joint involved
3=>2 joint involved
4severe arthritis of the entire paw and digits
An arthritis index for each rat is calculated by adding the scores for each of the 4 paws.
6. Swelling severity is also determined by quantitating the change in the paw volume, as measured by plethysmometry.

訂貨信息：4006551678

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20012	Immunization Grade Chick Type II Collagen, Solution	2775	chondrex
20021	Immunization Grade Bovine Type II Collagen	2775	chondrex
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20031	Immunization Grade Porcine Type II Collagen	2775	chondrex
20032	Immunization Grade Porcine Type II Collagen, Solution	2775	chondrex
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20051	Immunization Grade Human Type II Collagen	4590	chondrex
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20061	Immunization Grade Mouse Type II Collagen	2340	chondrex
20062	Immunization Grade Mouse Type II Collagen, Solution	2340	chondrex
20071	Immunization Grade Goat Type II Collagen	3075	chondrex
20081	Immunization Grade Sheep Type II Collagen	3075	chondrex
2011	ELISA Grade Chick Type II Collagen	2400	chondrex
2012	ELISA Grade Bovine Type II Collagen	2400	chondrex
2013	ELISA Grade Porcine Type II Collagen	2400	chondrex
2014	ELISA Grade Rat Type II Collagen	2910	chondrex
2015	ELISA Grade Human Type II Collagen	4785	chondrex
2016	ELISA Grade Mouse Type II Collagen	6750	chondrex
2017	ELISA Grade Monkey Type II Collagen	3000	chondrex
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