●問(wèn)題1

貨號(hào)：10-5000-02M

品名：Universal-CE Phosphoramidite -（10-5000）

品牌：Glen Research

原文：

Q:“10-5000-02M"?When coupling this product with hydroxyl groups, do we need to follow the same procedure as in chemical synthesis, i.e., first add it to the mixed solution of it and ETT, then cap and oxidize? Or can we directly immerse the solid-phase carrier with hydroxyl groups in water for 12 hours?

A:Yes, our product Universal-CE Phosphoramidite - (10-5000) should follow the same procedures as other the phosphoramidite reagents (Activation & coupling, capping, oxidation). Like other any phosphoramidites water should not be involved in the coupling of these reagents. Phosphoramidites are highly sensitive to water because it causes rapid hydrolysis destroying its ability to couple during oligonucleotide synthesis.

譯文：

問(wèn)：針對(duì)產(chǎn)品“10-5000-02M"，將其與羥基偶聯(lián)時(shí)，是否需要遵循化學(xué)合成的常規(guī)流程，即先將其加入該產(chǎn)物與ETT的混合溶液中，隨后進(jìn)行封端和氧化？還是說(shuō)我們可以直接將帶有羥基的固相載體浸入水中放置12小時(shí)？

答：是的，我們的產(chǎn)品 Universal-CE 亞磷酰胺 (10-5000) 應(yīng)遵循與其他亞磷酰胺試劑相同的操作流程（活化與偶聯(lián)、封端、氧化）。與其他所有亞磷酰胺一樣，此類(lèi)試劑的偶聯(lián)過(guò)程嚴(yán)禁接觸水。亞磷酰胺對(duì)水極其敏感，遇水會(huì)發(fā)生快速水解，從而喪失其在寡核苷酸合成中的偶聯(lián)能力。

●問(wèn)題2

貨號(hào)：NHS

品名：NHS試劑

品牌：Complement Technology

原文：

Q:Is this Human Serum derived from multiple donors or a single donor？Also, do you have any recent literature published using this product, or any protocols for its use?

A:No, you would have to look up paper, etc.  There should be some in the scientific literature databases.

譯文：

問(wèn)：這種人血清是來(lái)源于多名供體，還是單一供體？另外，請(qǐng)問(wèn)是否有近期發(fā)表的、使用了該產(chǎn)品的文獻(xiàn)，或者相關(guān)的使用方案？

答：沒(méi)有。你需要自行查閱論文等資料，科學(xué)文獻(xiàn)數(shù)據(jù)庫(kù)中應(yīng)該能找到一些相關(guān)信息。

●問(wèn)題3

貨號(hào)：A114

品名：C3b 1.0 mg/ml

品牌：Complement Technology

原文：

Q:What is the approximate percentage of activity for this product

A:There is no activiy.  It is C3b (fragment of C3).

譯文：

問(wèn)：請(qǐng)問(wèn)該產(chǎn)品的活性百分比約為多少？

答：無(wú)活性。其為 C3b（補(bǔ)體成分 C3 的裂解片段）。

●問(wèn)題4

貨號(hào)：Rb114/R114/M114

品名：Rabbit C3b/Rat C3b/Mouse C3b

品牌：Complement Technology

原文：

Q:What is the approximate percentage of activity for this product

A:C3b is a fragment of C3.  It has no activity.

For C3b there is no functional assay in the CoA. However, we do an in-house functional assay wherein using SDS-PAGE analysis we determine the cleavage of C3b to iC3b by FI in the presence of cofactor FH.

We do evaluate all our C3b preparations in an in-house functional assay but we do not report it. We analyze Factor I cleavage of C3b to iC3b in the presence of the cofactor Factor H by SDS-PAGE under reduced conditions. C3b under reduced conditions shows two bands of about 101,000 daltons for the alpha prime chain (α’-chain) and 75,000 daltons for the beta chain. In the presence of cofactor Factor H, Factor I cleaves the alpha prime chain of C3b (101,000 daltons) yielding two fragments (63,000 and 39,000 Da). Thus, when C3b is converted to iC3b we see the disappearance of the alpha prime chain and the appearance of two bands @ 63,000 and 39,000 Da in addition to the beta chain @ 75,000 daltons. Functional analysis of C3b indicates that our C3b preparations are > 90% active.

譯文：

問(wèn)：該產(chǎn)品的大致活性百分比是多少？

答：C3b 是 C3 的一個(gè)片段。它本身沒(méi)有（溶血）活性。

對(duì)于 C3b，在產(chǎn)品分析證書(shū)（CoA）中沒(méi)有功能性檢測(cè)。但是，我們會(huì)進(jìn)行一項(xiàng)內(nèi)部功能性檢測(cè)，即通過(guò) SDS-PAGE 分析，測(cè)定在輔因子 H（FH）存在下，因子 I（FI）將 C3b 裂解為 iC3b 的情況。

我們會(huì)通過(guò)這項(xiàng)內(nèi)部功能性檢測(cè)評(píng)估所有 C3b 制劑，但不將其報(bào)告在證書(shū)中。我們通過(guò)還原條件下的 SDS-PAGE，分析在輔因子 H 存在時(shí)因子 I 對(duì) C3b 的裂解情況。在還原條件下，C3b 顯示兩條條帶：約 101,000 道爾頓的 α' 鏈（α'-鏈）和 75,000 道爾頓的 β 鏈。在輔因子 H 存在的情況下，因子 I 會(huì)切割 C3b 的 α' 鏈（101,000 道爾頓），產(chǎn)生兩個(gè)片段（63,000 和 39,000 道爾頓）。因此，當(dāng) C3b 轉(zhuǎn)化為 iC3b 時(shí)，我們會(huì)觀察到 α' 鏈的消失，并出現(xiàn)位于 63,000 和 39,000 道爾頓處的兩條新條帶，此外還有位于 75,000 道爾頓處的 β 鏈。

對(duì) C3b 的功能分析表明，我們的 C3b 制劑活性 > 90%。

●問(wèn)題5

貨號(hào)：abx670024

品名：Human Complement C3b (C3b) Protein

品牌：Abbexa

原文：

Q:What is the approximate percentage of activity for this product?

A:The activity of this protein has not been tested.

譯文：

問(wèn)：請(qǐng)問(wèn)該產(chǎn)品的活性百分比約為多少？

答：該蛋白的活性尚未經(jīng)檢測(cè)。

更多產(chǎn)品信息，請(qǐng)聯(lián)系中國(guó)區(qū)域代理商：上海起發(fā)實(shí)驗(yàn)試劑有限公司

（注：本文內(nèi)容基于品牌公開(kāi)資料及行業(yè)常規(guī)信息整理，具體以品牌信息文檔為準(zhǔn)。）